A New MIC1-MAG1 Recombinant Chimeric Antigen Can Be Used Instead of the Toxoplasma gondii Lysate Antigen in Serodiagnosis of Human Toxoplasmosis
نویسندگان
چکیده
منابع مشابه
Production of MAG1 Antigen of Toxoplasma gondii in Escherichia coli
Introduction : Toxoplasmosis is a parasitic infection caused by the protozoan Toxoplasma gondii it leads to serious medical problems in congenitally-infected and immunocompromised individuals, while it is quite harmless in immunocompetent individuals. Toxoplasma tissue cyst matrix protein (MAG1) induces early humoral and cell-mediated immune responses. Previous studies suggested recombinant M...
متن کاملRecombinant MAG1 Protein of Toxoplasma gondii as a Diagnostic Antigen.
The aim of this study was to evaluate the potential diagnostic usefulness of the full-length recombinant Toxoplasma gondii MAG1 protein by determining the levels of specific IgM and IgG antibodies in mouse and human sera obtained from individuals with acute and chronic toxoplasmosis. The obtained results revealed that IgG antibodies against MAG1 are a sensitive and specific marker of T. gondii ...
متن کاملProduction of MAG1 Antigen of Toxoplasma gondii in Escherichia coli
Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect human and other warm-blooded animals. Although usually asymptomatic in immunocompetent individuals, toxoplasmosis may reactivate in immunocompromised patients (e.g. AIDS patients and organ transplant recipients) and cause severe diseases such as toxoplasmic encephalitis. Moreover, primary maternal infection can pu...
متن کاملChimeric antigens of Toxoplasma gondii: toward standardization of toxoplasmosis serodiagnosis using recombinant products.
We have evaluated the diagnostic utility of six antigenic regions of the Toxoplasma gondii MIC2, MIC3, M2AP, GRA3, GRA7, and SAG1 gene products, assembled in recombinant chimeric antigens by genetic engineering, in order to replace the soluble, whole-cell tachyzoite extract in serological assays. Serum samples from 100 adults with acquired T. gondii infection and from 30 infants born to mothers...
متن کاملproduction of mag1 antigen of toxoplasma gondii in escherichia coli
introduction : toxoplasmosis is a parasitic infection caused by the protozoan toxoplasma gondii it leads to serious medical problems in congenitally-infected and immunocompromised individuals, while it is quite harmless in immunocompetent individuals. toxoplasma tissue cyst matrix protein (mag1) induces early humoral and cell-mediated immune responses. previous studies suggested recombinant mag...
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ژورنال
عنوان ژورنال: Clinical and Vaccine Immunology
سال: 2012
ISSN: 1556-6811,1556-679X
DOI: 10.1128/cvi.05433-11